The Moby Project - a collaborative project for the container ecosystem to assemble container-based systems

🤖 Assemble, configure, and deploy autonomous AI Agents in your browser.

Learning operating system development using Linux kernel and Raspberry Pi

John the Ripper jumbo - advanced offline password cracker, which supports hundreds of hash and cipher types, and runs on many operating systems, CPUs, GPUs, and even some FPGAs

An assembled flutter application framework.

A container platform that needs no Kubernetes learning, Build, deploy, assemble, and manage apps on Kubernetes, no K8s expertise needed, all in a graphical platform.

SASM - simple crossplatform IDE for NASM, MASM, GAS and FASM assembly languages

An introduction to ARM64 assembly on Apple Silicon Macs

Low-latency machine code generation

Smarter YAML front matter parser, used by metalsmith, Gatsby, Netlify, Assemble, mapbox-gl, phenomic, vuejs vitepress, TinaCMS, Shopify Polaris, Ant Design, Astro, hashicorp, garden, slidev, saber, sourcegraph, and many others. Simple to use, and battle tested. Parses YAML by default but can also parse JSON Front Matter, Coffee Front Matter, TOML Front Matter, and has support for custom parsers. Please follow gray-matter's author: https://github.com/jonschlinkert

A curated list of Game Boy development resources such as tools, docs, emulators, related projects and open-source ROMs.

Get the rocks out of your socks! Assemble makes you fast at web development! Used by thousands of projects for rapid prototyping, themes, scaffolds, boilerplates, e-books, UI components, API documentation, blogs, building websites/static site generator, an alternative to Jekyll for gh-pages and more! Gulp- and grunt-friendly.

Visual Studio extension for assembly syntax highlighting and code completion in assembly files and the disassembly window

Reverse engineering framework in Python

Blazing fast and correct x86/x64 disassembler, assembler, decoder, encoder for Rust, .NET, Java, Python, Lua

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

A cross-platform x86 assembler with an Intel-like syntax

🤖 Assemble, configure, and deploy autonomous AI Agents in your browser.一键免费部署你的私人AutoGPT 网页应用

Reverse engineering tool for linux games

Keystone assembler framework: Core (Arm, Arm64, Hexagon, Mips, PowerPC, Sparc, SystemZ & X86) + bindings

188 handlebars helpers in ~20 categories. Can be used with Assemble, Ghost, YUI, express.js etc.

A JIT assembler for x86/x64 architectures supporting FPU, MMX, SSE (1-4), AVX (1-2, 512), APX, and AVX10.2

An updated version of GBDK, C compiler, assembler, linker and set of libraries for the Nintendo Gameboy, Nintendo Entertainment System, Sega Master System, Sega Game Gear.

Java decompiler, assembler, and disassembler

x86-64 assembler embedded in Python

Multi-architecture assembler for IDA Pro. Powered by Keystone Engine.

API and CLI for generating a markdown TOC (table of contents) for a README or any markdown files. Uses Remarkable to parse markdown. Used by NASA/openmct, Prisma, Joi, Mocha, Sass, Prettier, Orbit DB, FormatJS, Raneto, hapijs/code, webpack-flow, docusaurus, release-it, ts-loader, json-server, reactfire, bunyan, husky, react-easy-state, react-snap, chakra-ui, carbon, alfresco, repolinter, Assemble, Verb, and thousands of other projects.

A repo of small demos that assemble some of the well-known design patterns in Unity development to support the ebook "Level up your code with game programming patterns"

A highly impartial suite of React components that can be assembled by the consumer to create a carousel with almost no limits on DOM structure or CSS styles. If you're tired of fighting some other developer's CSS and DOM structure, this carousel is for you.

Parallel OS, with GUI, Terminal, OO Assembler, Class libraries, C-Script compiler, Lisp interpreter and more...