Code for inferring fitnesses from barcode abundance data

Code/data used for applying SVM to high-speed imaging data of a pain assay

The one phase association non-linear regression equation best models protein standards assayed via the Bradford technique. Unknown protein samples can be interpolated with greater accuracy.

Cellular Morphological Features Improve Prediction of Cytotoxicity- and Proliferation-Related Assay Endpoints when Augmented with Molecular Fingerprints

PB1flank-eGFP neutralization assay details

Processes assay screening data outputted from Perkin-Elmer Envision plate readers for compatibility to upload into a database for further analyses and comparisons across experiments.

PISA-M(P): Prediction of Interference with Specific assay Methods

Evaluation framework for Pydantic AI agents

This is a R Shiny application to visualise proliferation curves

PHP SDK for Assay Depot JSON API.

A simple interface to help scientists design experiments using an assay plate, a rectangular device with wells containing sets of reagents (e.g., https://www.bio-rad.com/en-us/product/384-well-pcr-plates?ID=OCH4UM15)

No description available

A modified PMR assay for zebrafish embryos using a Zantiks MWP machine and a 96-well custom-made embryo plate.

Quantify colony growth on solid media plates under different environment factors from pictures taken from the incubation at regular intervals.

No description available

No description available

No description available

This is a repository with all data and code used for the article: "A label-free high-throughput protein solubility assay and its application to Aβ40" DOI: https://doi.org/10.1016/j.bpc.2023.107165

Automated quality control tool for high-content imaging data by building 2D prediction intervals on reference biosignatures

No description available

Code published with the paper Zhu S.I., McCullough M., Pujic J., Sibberas J., Sun, B. Darveniza T., Bucknall B., Avitan L., and Goodhill, G.J. "fmr1 mutation alters the early development of sensory coding and hunting and social behaviors in larval zebrafish". Journal of Neuroscience 2022

This is a BioTek Gen5 plate reader data processing script.

This repository has all the code and data used to develop and analyze the Pycnopodia helianthoides eDNA qPCR assay

No description available

This archive contains all the scripts needed to process the data presented in the manuscript titled ''Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding''

A simple example of image segmentation using Digital Image Processing

No description available

Objective. Rhizoma Anemarrhenae, the dried rhizome of Anemarrhena asphodeloides, has been widely used in China as a traditional medicine for thousands of years. This study was designed to explore the protective effects and mechanisms of the aqueous extracts from Anemarrhena asphodeloides Bunge in mice with allergic contact dermatitis (ACD). Methods. In the present study, HPLC-ELSD was used to analysis the principal component of Anemarrhena asphodeloides water extracts (AAWE). Pharmacodynamic effects of AAWE were evaluated in ACD mice induced by repeated application of DNFB. Ear swelling and spleen index were estimated. Ear tissues histopathological changes were observed under a light microscope after stained with H&E. The serum levels of inflammatory factors (TNF-α and IFN-γ) in mice were determined via Enzyme-linked immunosorbent assay (ELISA). To further understand the mechanism underlying the inhibitory effect of AAWE on mice with ACD, we investigated whether AAWE could influence the expression of MAPKs and PI3K/AKT signal pathways by immune-histochemical, Western blotting (WB), and Real-time polymerase chain reaction (RT-PCR). Flow cytometry analyzed NK lymphocytes amount in peripheral blood. Results. 7 compounds were identified in AAWE. Oral administration of AAWE could significantly inhibit the production of inflammatory factors such as the serum levels of TNF-α and IFN-γ in mice with ACD induced by DNFB. Also, AAWE treatment effectively prevented ear swelling and reduced spleen indexes in mice. Furthermore, the results of HE staining indicated that AAWE treatment substantially improved the skin microstructure of mice with ACD. The results of flow cytometry showed that AAWE obviously inhibited NK cell proliferation. In addition, the mRNA and protein expression levels of PI3K and AKT were inhibited in the ears of ACD mice. Lastly, the results from immune-histochemical method showed that AAWE had a remarkable down-regulation on the PI3K and p-AKT level. Conclusion. This study demonstrated that AAWE could inhibit inflammatory reactions and alleviate ACD in mice by inhibiting PI3K/AKT signaling pathway, which provided novel insight into the molecular mechanism of AAWE in relieving ACD.

Automated code review harness for OpenCode native Ralph Loop — no external orchestrator required

Plan experiments with AssayPlate!