Found 74,093 repositories(showing 30)
apify
Crawlee—A web scraping and browser automation library for Node.js to build reliable crawlers. In JavaScript and TypeScript. Extract data for AI, LLMs, RAG, or GPTs. Download HTML, PDF, JPG, PNG, and other files from websites. Works with Puppeteer, Playwright, Cheerio, JSDOM, and raw HTTP. Both headful and headless mode. With proxy rotation.
moonD4rk
Extract and decrypt browser data, supporting multiple data types, runnable on various operating systems (macOS, Windows, Linux).
apify
Crawlee—A web scraping and browser automation library for Python to build reliable crawlers. Extract data for AI, LLMs, RAG, or GPTs. Download HTML, PDF, JPG, PNG, and other files from websites. Works with Parsel, BeautifulSoup, Playwright, and raw HTTP. Both headful and headless mode. With proxy rotation.
cloudquery
Data pipelines for cloud config and security data. Build cloud asset inventory, CSPM, FinOps, and vulnerability management solutions. Extract from AWS, Azure, GCP, and 70+ cloud and SaaS sources.
nickscamara
An open source deep research clone. AI Agent that reasons large amounts of web data extracted with Firecrawl
bjesus
Swiss-army tool for scraping and extracting data from online assets, made for hackers
extract internal monitoring data from application logs for collection in a timeseries database
camelot-dev
A Python library to extract tabular data from PDFs
gosom
scrape data from Google Maps. Extracts data such as the name, address, phone number, website URL, rating, reviews number, latitude and longitude, reviews,email and more for each place
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
ignis-sec
A collection of all the data i could extract from 1 billion leaked credentials from internet.
pydata
Extract data from a wide range of Internet sources into a pandas DataFrame.
blockchain-etl
Python scripts for ETL (extract, transform and load) jobs for Ethereum blocks, transactions, ERC20 / ERC721 tokens, transfers, receipts, logs, contracts, internal transactions. Data is available in Google BigQuery https://goo.gl/oY5BCQ
automeris-io
Computer vision assisted tool to extract numerical data from plot images.
apache
Apache DevLake is an open-source dev data platform to ingest, analyze, and visualize the fragmented data from DevOps tools, extracting insights for engineering excellence, developer experience, and community growth.
oxylabs
Free Trial Amazon Scraper API for extracting search, product, offer listing, reviews, question and answers, best sellers and sellers data.
oxylabs
Crawl a website starting from a URL, find relevant pages, and extract data – all guided by your natural language prompt.
any4ai
AnyCrawl 🚀: A Node.js/TypeScript crawler that turns websites into LLM-ready data and extracts structured SERP results from Google/Bing/Baidu/etc. Native multi-threading for bulk processing.
The process of extracting product data from Amazon using Python, including titles, ratings, prices, images, and descriptions.
smalot
PdfParser, a standalone PHP library, provides various tools to extract data from a PDF file.
omkarcloud
Google Maps Scraper & Lead Generation Tool. Extract 50+ data points including business emails, phone numbers, and social profiles. Includes enrichment features, API access, and no recurring fees
oxylabs
Learn step-by-step how to scrape Google Trends data and make a result comparison using Python and Oxylabs SERP API. Extract keywords, their popularity, breakdown by region, related queries, and more.
WZBSocialScienceCenter
A set of tools for extracting tables from PDF files helping to do data mining on (OCR-processed) scanned documents.
invoice-x
Extract structured data from PDF invoices
xaitax
This tool extracts and displays data from the Recall feature in Windows 11, providing an easy way to access information about your PC's activity snapshots.
TeamNewPipe
NewPipe's core library for extracting data from streaming sites
camelot-dev
A web interface to extract tabular data from PDFs
devnied
A Java library used to read and extract data from NFC EMV credit cards (Android/PCSC).
capitalone
What's in your data? Extract schema, statistics and entities from datasets
paradigmxyz
cryo is the easiest way to extract blockchain data to parquet, csv, json, or python dataframes