Found 63,498 repositories(showing 30)
pppscn
短信转发器——监控Android手机短信、来电、APP通知,并根据指定规则转发到其他手机:钉钉群自定义机器人、钉钉企业内机器人、企业微信群机器人、飞书机器人、企业微信应用消息、邮箱、bark、webhook、Telegram机器人、Server酱、PushPlus、手机短信等。包括主动控制服务端与客户端,让你轻松远程发短信、查短信、查通话、查话簿、查电量等。(V3.0 新增)PS.这个APK主要是学习与自用,如有BUG请提ISSUE,同时欢迎大家提PR指正
sshuttle
Transparent proxy server that works as a poor man's VPN. Forwards over ssh. Doesn't require admin. Works with Linux and MacOS. Supports DNS tunneling.
89luca89
Use any linux distribution inside your terminal. Enable both backward and forward compatibility with software and freedom to use whatever distribution you’re more comfortable with. Mirror available at: https://gitlab.com/89luca89/distrobox
harthur
Simple feed-forward neural network in JavaScript
zyronon
Practice English, one strike, one step forward; 练习英语,一次敲击,一点进步;
bndr
pipreqs - Generate pip requirements.txt file based on imports of any project. Looking for maintainers to move this project forward.
wangyu-
A Tunnel which Improves your Network Quality on a High-latency Lossy Link by using Forward Error Correction, possible for All Traffics(TCP/UDP/ICMP)
productdevbook
A powerful cross-platform port management tool for developers. Monitor ports, manage Kubernetes port forwards, integrate Cloudflare Tunnels, and kill processes with one click.
FastForwardTeam
Don't waste your time with compliance. FastForward automatically skips annoying link shorteners.
nadoo
glider is a forward proxy with multiple protocols support, and also a dns/dhcp server with ipset management features(like dnsmasq).
andremion
From UI Proposal to Code :notes::arrow_forward:
abhinavsingh
💫 Ngrok FRP Alternative • ⚡ Fast • 🪶 Lightweight • 0️⃣ Dependency • 🔌 Pluggable • 😈 TLS interception • 🔒 DNS-over-HTTPS • 🔥 Poor Man's VPN • ⏪ Reverse & ⏩ Forward • 👮🏿 "Proxy Server" framework • 🌐 "Web Server" framework • ➵ ➶ ➷ ➠ "PubSub" framework • 👷 "Work" acceptor & executor framework
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
facebookresearch
MapAnything: Universal Feed-Forward Metric 3D Reconstruction
libffcv
FFCV: Fast Forward Computer Vision (and other ML workloads!)
ehForwarderBot
An extensible message tunneling chat bot framework. Delivers messages to and from multiple platforms and remotely control your accounts.
keenthemes
Design-forward shadcn kit for interfaces that stand out. 1000+ free patterns!
gravitational
Command line tool to share your UNIX terminal and forward local TCP ports to people you trust.
mitsuba-renderer
Mitsuba 3: A Retargetable Forward and Inverse Renderer
wangyu-
A VPN Designed for Lossy Links, with Build-in Forward Error Correction(FEC) Support. Improves your Network Quality on a High-latency Lossy Link.
forwardemail
Repository archived and moved to monorepo
thomseddon
Minimal forward authentication service that provides Google/OpenID oauth based login and authentication for the traefik reverse proxy
clarketm
A list of free, public, forward proxy servers. UPDATED DAILY!
bittentech
This is a resource factory for anyone looking forward to starting bug hunting and would require guidance as a beginner.
casadi
CasADi is a symbolic framework for numeric optimization implementing automatic differentiation in forward and reverse modes on sparse matrix-valued computational graphs. It supports self-contained C-code generation and interfaces state-of-the-art codes such as SUNDIALS, IPOPT etc. It can be used from C++, Python or Matlab/Octave.
glouw
A tiny neural network library
mitsuba-renderer
Mitsuba 2: A Retargetable Forward and Inverse Renderer
A forward proxy module for CONNECT request handling
cossacklabs
Easy to use cryptographic framework for data protection: secure messaging with forward secrecy and secure data storage. Has unified APIs across 14 platforms.
editso
一款体积小, 快速, 稳定, 高效, 轻量的内网穿透, 端口转发工具 支持多连接,级联代理,传输加密 (A small volume, fast, stable, efficient, and lightweight intranet penetration, port forwarding tool supports multiple connections, cascading proxy, and transmission encryption)