Streisand sets up a new server running your choice of WireGuard, OpenConnect, OpenSSH, OpenVPN, Shadowsocks, sslh, Stunnel, or a Tor bridge. It also generates custom instructions for all of these services. At the end of the run you are given an HTML file with instructions that can be shared with friends, family members, and fellow activists.

🏎 A set of primitives to build simple, flexible, WAI-ARIA compliant React autocomplete, combobox or select dropdown components.

A curated set of 200+ plug-and-play n8n workflows that fuse classic automation with today’s AI stack—vector DBs, embeddings, and LLMs. Import any JSON, add your creds, hit Activate, and you’re live. Built to demo, prototype, or drop straight into production.

MapDB provides concurrent Maps, Sets and Queues backed by disk storage or off-heap-memory. It is a fast and easy to use embedded Java database engine.

Visual Studio Code Remote Development: Open any folder in WSL, in a Docker container, or on a remote machine using SSH and take advantage of VS Code's full feature set.

A very extensive set of extension methods that allow you to more naturally specify the expected outcome of a TDD or BDD-style unit tests. Targets .NET Framework 4.7, as well as .NET Core 2.1, .NET Core 3.0, .NET 6, .NET Standard 2.0 and 2.1. Supports the unit test frameworks MSTest2, NUnit3, XUnit2, MSpec, and NSpec3.

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

🔐 Securely share sensitive information with automatic expiration & deletion after a set number of views or duration. Track who, what and when with full audit logs.

Lecture Notes for Linear Algebra Featuring Python. This series of lecture notes will walk you through all the must-know concepts that set the foundation of data science or advanced quantitative skillsets. Suitable for statistician/econometrician, quantitative analysts, data scientists and etc. to quickly refresh the linear algebra with the assistance of Python computation and visualization.

A small set of tools to convert packets from capture files to hash files for use with Hashcat or John the Ripper.

This application gives Mac users in enterprise environments control over the administration of their machines by elevating their access level to administrator privileges on macOS. Users can set a timeframe in the application's settings to perform specific tasks, such as installing or uninstalling applications.

Your memories are in ChatGPT... But nowhere else. Universal Memory MCP makes your memories available to every single LLM. No logins or paywall. One command to set it up.

Multitrack Web Audio editor and player with canvas waveform preview. Set cues, fades and shift multiple tracks in time. Record audio tracks or provide audio annotations. Export your mix to AudioBuffer or WAV! Add effects from Tone.js. Project inspired by Audacity.

Carefully Designed Icon Library. MingCute is a set of simple and exquisite open-source icon library. Whether you're a designer or a developer, it's perfect for use in web and mobile.

[UNMAINTAINED] Replaces references to non-optimized scripts or stylesheets into a set of HTML files (or any templates/views)

A multi-platform bug bounty toolkit that can be installed on Debian/Ubuntu or set up with Docker.

🎉 A beautiful Kotlin app to remember birthdays and events without having to open Facebook, set alarms or rely on Google Calendar

BaseAnimation network Android animation set, custom controls, nearly 200 kinds of source code！ BaseAnimation, if a new version is updated automatically to remind everyone, I hope everyone will contribute their animated XML files or other source, together to create this open source app！

Search Google/Bing/Ecosia/DuckDuckGo/Yandex/Yahoo for a search term (dork) with a default set of websites, bug bounty programs or custom collection.

:wrench: :hammer: A set of PowerShell functions you might use to enhance your own functions and scripts or to facilitate working in the console. Most should work in both Windows PowerShell and PowerShell 7, even cross-platform. Any operating system limitations should be handled on a per command basis. The Samples folder contains demonstration script files

Test DPC is a sample device policy controller for use with Android Enterprise. It gives developers the ability to see how their app will behave in a managed context such as device owner or within a managed profile. Users can set up a work profile, enable work apps, set applications restrictions, manage security polices, and much more. The app also serves as a implementation reference for other DPCs

Use full GM set of musical instruments to play MIDI and single sounds or effects. Support for reverberation and equaliser. No plugins, no Flash. Pure HTML5 implementation compatible with desktop and mobile browser. See live examples.

Get, set, or delete a property from a nested object using a dot path

Use glob patterns to watch file sets and run a command when anything is added, changed or deleted.

Tuplex is a parallel big data processing framework that runs data science pipelines written in Python at the speed of compiled code. Tuplex has similar Python APIs to Apache Spark or Dask, but rather than invoking the Python interpreter, Tuplex generates optimized LLVM bytecode for the given pipeline and input data set.

Kuwala is the no-code data platform for BI analysts and engineers enabling you to build powerful analytics workflows. We are set out to bring state-of-the-art data engineering tools you love, such as Airbyte, dbt, or Great Expectations together in one intuitive interface built with React Flow. In addition we provide third-party data into data science models and products with a focus on geospatial data. Currently, the following data connectors are available worldwide: a) High-resolution demographics data b) Point of Interests from Open Street Map c) Google Popular Times

A complete set of functions to read and write H.264 video bitstreams, in particular to examine or modify headers.

HMGLTransitions is set of classes which can animate transition between two UIViews, or presentation / dismissing of UIViewControllers.

A OAuth2 server library, for use in combination with actix or other frontends, featuring a set of configurable and pluggable backends.

Dito como o maior e melhor Setup de soluções Open Source, 100% Gratuito.