A complete computer science study plan to become a software engineer.

什么？你敢放心的把后背交给 AI? 我赌你不敢，那就来学学 AI 时代最安全的语言吧(Python无法战胜！)。本书拥有全面且深入的讲解、生动贴切的示例、德芙般丝滑的内容，这可能是目前最用心的 Rust 中文学习教程 / Book

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:boy: :girl: Technical-Interview guidelines written for those who started studying programming. I wish you all the best. :space_invader:

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This repository contains a 90-day cybersecurity study plan, along with resources and materials for learning various cybersecurity concepts and technologies. The plan is organized into daily tasks, covering topics such as Network+, Security+, Linux, Python, Traffic Analysis, Git, ELK, AWS, Azure, and Hacking. The repository also includes a `LEARN.md

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Learning Rust By Practice, narrowing the gap between beginner and skilled-dev through challenging examples, exercises and projects.

Weekly Go Online Meetup via Bilibili｜Go 夜读｜通过 bilibili 在线直播的方式分享 Go 相关的技术话题，每天大家在微信/telegram/Slack 上及时沟通交流编程技术话题。

This repository contains a curated collection of 300+ case studies from over 80 companies, detailing practical applications and insights into machine learning (ML) system design. The contents are organized to help you easily find relevant case studies based on industry or specific ML use cases.

:anchor: 我的游戏程序员生涯的读书笔记合辑。你可以把它看作一个加强版的Blog。涉及图形学、实时渲染、编程实践、GPU编程、设计模式、软件工程等内容。Keep Reading , Keep Writing , Keep Coding.

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ffffffff0x 团队维护的安全知识框架,内容包括不仅限于 web安全、工控安全、取证、应急、蓝队设施部署、后渗透、Linux安全、各类靶机writup

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Complete Practical Study Plan to become a successful cybersecurity engineer based on roles like Pentest, AppSec, Cloud Security, DevSecOps and so on...

Studying for a tech interview sucks. Here's an open source cheat sheet to help

The perfect emulation setup to study and develop the Linux kernel, kernel modules, QEMU, gem5 and x86_64, ARMv7 and ARMv8 userland and baremetal assembly, ANSI C, C++ and POSIX. GDB step debug and KGDB just work. Powered by Buildroot and crosstool-NG. Highly automated. Thoroughly documented. Automated tests. "Tested" in an Ubuntu 24.04 host.

一份硬核(hardcore)计算机科学CS自学计划，偏向软件工程和系统架构方向

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该仓库主要记录 NLP 算法工程师相关的顶会论文研读笔记

Android study notes.

学习记录

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.