Useful tool to track location or mobile number

A remote debugging platform you'll definitely find useful. Lightweight, cross-platform, out-of-box debugging tool

Fortio load testing library, command line tool, advanced echo server and web UI in go (golang). Allows to specify a set query-per-second load and record latency histograms and other useful stats.

An effort to build a single place for all useful android and iOS security related stuff. All references and tools belong to their respective owners. I'm just maintaining it.

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

A useful tools or tips list for mobile web application developing

JSCAD is an open source set of modular, browser and command line tools for creating parametric 2D and 3D designs with JavaScript code. It provides a quick, precise and reproducible method for generating 3D models, and is especially useful for creating ready-to-print 3D designs.

A reviewed list of useful PHP static analysis tools

Useful links, scripts, tools and best practice for Microsoft SQL Server Database

macro_pack is a tool by @EmericNasi used to automatize obfuscation and generation of Office documents, VB scripts, shortcuts, and other formats for pentest, demo, and social engineering assessments. The goal of macro_pack is to simplify exploitation, antimalware bypass, and automatize the process from malicious macro and script generation to final document generation. It also provides a lot of helpful features useful for redteam or security research.

A collection of most useful osint tools for SOCINT.

possibly useful tools for the Internet

Assorted useful tools, almost entirely generated using LLMs

CrimeFlare is a useful tool for bypassing websites protected by CloudFlare WAF, with this tool you can easily see the real IP of websites that have been protected by CloudFlare. The resulting information is certainly very useful for conducting further penetration testing, and analyzing websites with the same server.

Set of useful android network tools

org-mode modules for citations, cross-references, bibliographies in org-mode and useful bibtex tools to go with it.

Strategies to Gekko trading bot with backtests results and some useful tools.

Searchlogic provides object based searching, common named scopes, and other useful tools.

Useful snippets / tools for using WSL2 as a development environment

The missing set of useful tools for Postgres DBAs and all engineers

Makani was a project to develop a commercial-scale airborne wind turbine, culminating in a flight test of the Makani M600 off the coast of Norway. All Makani software has now been open-sourced. This repository contains the working Makani flight simulator, controller (autopilot), visualizer, and command center flight monitoring tools. Additionally, almost all avionics firmware is also included, albeit potentially not in a buildable state, due to the removal of some third-party proprietary code. We hope that this code will be inspirational and useful to the kite-based windpower and wider communities.

一个有点好用的信息收集工具。A somewhat useful information gathering tool.

A useful tool for Android Developers, which shows the package name and class name of current activity.

Tool for discovering the origin host behind a reverse proxy. Useful for bypassing cloud WAFs!

Little bits of information that you may find useful when interacting with Expo tools and service. Append the markdown filename to https://expo.fyi/ to get a quick link to it.

A collection of useful online web development tools

A convenient and useful tool for migrating data between redis group.

A list of useful Apache NiFi resources, processor bundles and tools

Pytools: Some useful tools written by pure python.

ArscBlamer is a command-line tool that can parse an Android app's resources.arsc file and extract useful, actionable information about its contents