Found 13,867 repositories(showing 30)
MichaelCade
This repository started out as a learning in public project for myself and has now become a structured learning map for many in the community. We have 3 years under our belt covering all things DevOps, including Principles, Processes, Tooling and Use Cases surrounding this vast topic.
Orama-Interactive
Unleash your creativity with Pixelorama, a powerful and accessible open-source pixel art multitool. Whether you want to create sprites, tiles, animations, or just express yourself in the language of pixel art, this software will realize your pixel-perfect dreams with a vast toolbox of features. Available on Windows, Linux, macOS and the Web!
esbatmop
MNBVC(Massive Never-ending BT Vast Chinese corpus)超大规模中文语料集。对标chatGPT训练的40T数据。MNBVC数据集不但包括主流文化,也包括各个小众文化甚至火星文的数据。MNBVC数据集包括新闻、作文、小说、书籍、杂志、论文、台词、帖子、wiki、古诗、歌词、商品介绍、笑话、糗事、聊天记录等一切形式的纯文本中文数据。
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
zoriya
A portable and vast media library solution.
libtom
LibTomCrypt is a fairly comprehensive, modular and portable cryptographic toolkit that provides developers with a vast array of well known published block ciphers, one-way hash functions, chaining modes, pseudo-random number generators, public key cryptography and a plethora of other routines.
hassanhabib
This is The Standard. A collection of decades of experience in the engineering industry. I authored it to help you navigate the vast ocean of knowledge. The Standard is not perfect and never will be, and it reflects the ongoing evolution of the engineering industry. While one person may write it, it is the collection of thoughts from hundreds of engineers I've had the honor to interact with and learn from throughout my life.
ai-dock
ComfyUI docker images for use in GPU cloud and local environments. Includes AI-Dock base for authentication and improved user experience.
fluid-player
Fluid Player - an open source VAST compliant HTML5 video player
Lewis-Stuart-11
3DGS-to-PC: 3D Gaussian Splatting to Dense Point Clouds [3D-VAST: ICCVW 2025]
openplayerjs
Lightweight HTML5 video/audio player with smooth controls and ability to play VAST/VMAP/SIMID/non-linear ads
IndoNLP
The first-ever vast natural language processing benchmark for Indonesian Language. We provide multiple downstream tasks, pre-trained IndoBERT models, and a starter code! (AACL-IJCNLP 2020)
ejoy
A game demo for Ant engine
kangpeilun
This is an unofficial Implementation
alohe
Avatars is a collection of diverse free avatar images from across the web, designed to add personality to your projects. With a vast selection of vibrant illustrations, you can enhance your projects in seconds.
trailofbits
VAST is an experimental compiler pipeline designed for program analysis of C and C++. It provides a tower of IRs as MLIR dialects to choose the best fit representations for a program analysis or further program abstraction.
reticulatedpines
A Git based version of Magic Lantern, for those unwilling or unable to work using Mercurial. The vast majority of branches have been removed, with those thought to be important brought in individually and merged.
p0dalirius
FindUncommonShares is a Python script allowing to quickly find uncommon shares in vast Windows Domains, and filter by READ or WRITE accesses.
dailymotion
VAST (up to 6) parsing library for JavaScript
wcampbell0x2a
GDB TUI Dashboard for the understanding of vast knowledge
CASIA-IVA-Lab
[NIPS2023] Code and Model for VAST: A Vision-Audio-Subtitle-Text Omni-Modality Foundation Model and Dataset
MailOnline
video.js vast plugin
niklas2902
Python scripting for Godot. This GDExtension plugin allows you to use Python like GDScript, accessing the Godot engine's features and benefiting from Python's vast ecosystem.
enricocid
📱 Minimal app to apply wallpapers from a vast (400+) collection of vector graphics 🙃 :trollface:
theonion
A VideoJS plugin for VAST
chrislusf
A distributed key-value store. On Disk. Able to grow or shrink without service interruption.
InteractiveAdvertisingBureau
Sample VAST Tag Use cases
gwen001
A vast collection of security tools and resources curated by the community.
KeeyanGhoreshi
A list of inputs that will beat the vast majority of Pokemon Firered games
Shadez
The World of Warcraft Armory is a vast searchable database of information for World of Warcraft - taken straight from the real servers and presented in a user-friendly interface. Since the Armory pulls its data from the actual game servers, it is the most comprehensive and up-to-date database on the characters, arena teams, guilds, and items of World of Warcraft in existence.