CoMOLA is a generic python tool for Constrained Multi-objective Optimization of Land use Allocation. It offers a framework to explore a landscape’s potential for ecosystem service supply and biodiversity conservation considering land use conversion and areal coverage constraints. CoMOLA can be used immediately by inputting a raster map representing the status-quo land use, ready-to-run models written in R including their input data, and (optional) information on constraints. The end product is a set of pareto-optimal solutions (land use maps) from which decision makers can discuss and select appropriate solutions according to their preferences.